A theoretical estimate for nucleotide sugar demand towards Chinese Hamster Ovary cellular glycosylation

Glycosylation greatly influences the safety and efficacy of many of the highest-selling recombinant therapeutic proteins (rTPs). In order to define optimal cell culture feeding strategies that control rTP glycosylation, it is necessary to know how nucleotide sugars (NSs) are consumed towards host cell and rTP glycosylation. Here, we present a theoretical framework that integrates the reported glycoproteome of CHO cells, the number of N-linked and O-GalNAc glycosylation sites on individual host cell proteins (HCPs), and the carbohydrate content of CHO glycosphingolipids to estimate the demand of NSs towards CHO cell glycosylation. We have identified the most abundant N-linked and O-GalNAc CHO glycoproteins, obtained the weighted frequency of N-linked and O-GalNAc glycosites across the CHO cell proteome, and have derived stoichiometric coefficients for NS consumption towards CHO cell glycosylation. By combining the obtained stoichiometric coefficients with previously reported data for specific growth and productivity of CHO cells, we observe that the demand of NSs towards glycosylation is significant and, thus, is required to better understand the burden of glycosylation on cellular metabolism. The estimated demand of NSs towards CHO cell glycosylation can be used to rationally design feeding strategies that ensure optimal and consistent rTP glycosylation

Ambigüedad y Promiscuidad

En 1919 William Randolph Hearst inicia la construcción de su suntuoso castillo en California a partir de una cantidad enorme de piezas expoliadas en Europa, dando como resultado una asombrosa reconstrucción y montaje de piezas venidas de diferentes épocas, estilos y culturas materiales. La arquitectura funciona por tanto como soporte para los ensamblajes culturales y materiales de diferentes tiempos. El trabajo que llevamos produciendo, en una red promiscua de colaboraciones, explora la aglutinación como espacio para la producción de comunidades y el desarrollo de los deseos de las mismas. Describiremos la confianza en la arquitectura como un montaje heterogéneo de realidades, que necesita ser pensada desde sus soportes hasta los ensamblajes espaciales, programáticos, materiales y temporales.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

Flannery O'Connor and her standpoint on the problem of evilness

Draf

Cuerpo común y guerra de los afectos. Coreografías globales y cuerpos en serie del Afectocapital

El ensayo propone un marco de análisis de las nuevas formas de operación del poder como dispositivos de producción afectiva y su operación a través del paradigma del pancoreográfico, o conjunto de dispositivos de distribución global de coreografías estándar. Plantea una redefinición de la corporalidad como campo de fuerzas relacionales en movimiento y la posibilidad de desarrollar resistencias a los mecanismos de estandarización de cuerpos del afectocapital, a través de nuevas tecnologías del movimiento a través del paradigma del cuerpo pos-anatómico.This essay proposes a frame of analysis of the new forms of power operating, as the devices of affective production trhough the paradygm of panchoreographics, or the sum of all the devices of global distribution of standard choreographies. It proposes a redefinition of corporality as a field of related sthrenghts in movement, and the possibility of the standarisation of the bodies in the affection capital, through the new technologies of movement of the paradigm of a post- anatomical body.Cet essai propose un plan de analyse des nouvelles formes d’opération du pouvoir en tant que dispositifs de production affective à travers du paradigme panchoréographique, le conjoint des dispositifs de distribution globalle de choréographies standard. Il presente une redefinition de la corporéité en tant que champ des forces de relation en mouvement, et la possibilité de dévélopper des ressistances aux méchanismes de standarisation des corps du affect-capital, à travers les nouvelles technologies du movement du paradygme du corps post-anatomique

Tiempo y eficacia

El modo de vida actual fomenta la instantaneidad y la prisa. En el periodismo, ligado por definición a la noticia y la actualidad, este apresuramiento se hace especialmente palmario, en detrimento, demasiadas veces, del análisis y la reflexión. El periodismo cultural, por la naturaleza de su propio objeto (la cultura, el conocimiento, las artes), aparece como un reducto de libertad de pensamiento y expresión en que la servidumbre a la inmediatez es menor, pero con demasiada frecuencia la industria cultural pesa más que la cultura a secas, y el criterio de oportunidad comercial se impone. El presente artículo analiza esta situación y aboga por valores como la reflexión, la elaboración y la vocación minoritarias como antídoto contra la mercantilización y banalización de la prensa cultural

Efficient processing of an antigenic sequence for presentation by MHC class I molecules depends on its neighboring residues in the protein

Processing of endogenously synthesized proteins generates short peptides that are presented by MHC class I molecules to CD8 T lymphocytes. Here it is documented that not only the sequence of the presented peptide but also the residues by which it is flanked in the protein determine the efficiency of processing and presentation. This became evident when a viral sequence of proven antigenicity was inserted at different positions into an unrelated carrier protein. Not different peptides, but different amounts of the antigenic insert itself were retrieved by isolation of naturally processed peptides from cells expressing the different chimeric proteins. Low yield of antigenic peptide from an unfavorable integration site could be overcome by flanking the insert with oligo-alanine to space it from disruptive neighboring sequences. Notably, the degree of protection against lethal virus disease related directly to the amount of naturally processed antigenic peptide

Undoing Anatomy: Resisting Global Choreographies in the Capitalism of Affects

Underlying the imaginaries staged and projected in texts, theater pieces, video, cinema and publicity there are certain anatomic constructions of the senses that are the conditions of possibility for those imaginaries. Such sensory anatomies underlie implicit power mechanisms in late cap­italist societies, which operate at the level of the production of affect and desire of consumers as well as of the concealment of global violence through which the economic system operates. I will propose a frame­work of analysis of such power operations through the analysis of move­ment, and more precisely of what I will call the panchoreographic: a set of technological devices of global distribution that disseminate discrete, standard choreographies in bodies, thus contributing to the production and dissemination of standard affects. Finally, I will show some exam­ples of the work developed by REVERSO in recent years that aims at the radical redefinition of the sensory anatomies that underlie media culture and information society, aiming at the production of a post-anatomical relational body

Water. Object of Desire and Source of Conflict in Castile in the Late Middle Ages

Producción CientíficaTo the medieval mind, water was a prized asset and one highly sought after both for the personal use to which it could be put and for the benefits it afforded vis-à-vis enhancing the reputation of those able to enjoy it. As a result, late Middle Age Castilian society strove not only to secure itself a supply of water for its survival but also to enhance its prestige. This led to the emergence of conflicting interests surrounding water, sparking clashes involving those in authority, cities, and private citizens. The present article explores these issues in the context of lower medieval Castilian urban society, focusing on the reasons underlying the social rivalry concerning water and the measures taken to stave off such conflict.Ministerio de Economía y Competitividad (HAR2012-32264